Disputed more than: Han Chunyu discloses a new experimental method, step change-gene, DNA-IT information
Han Chunyu storm of public opinion gradually when the fermentation, on August 8, Han Chunyu submitted to the non-profit grain shared information Addgene new experimental methods, adding a number of problems that should be noticed.
▲ Photo caption: Korea spring
China Youth daily reporter seen in the Addgene website, new experimental methods are divided into cells, plasmid/gDNA cotransfection, genome of the three parts, some details in the paper published in nature-life science is different.
According to "Science Americans" Chinese version global science of micro-letter public, "Science circle" introduced, new experiment steps has several at change, which including "serum brand by Hyclone change for Gibco", "dissolved and diluted quality grain and the gDNA of buffer liquid change for water (pH 8.0)", "recommends in quality grain/gDNA total turned dye of 8 hours, and 12 hours or 24 hours Hou, fill turned a times gDNA (old version method in the only mentioned has '24 hours ')", content.
In addition, Han Chunyu also on the new test method 4 are listed in note, including "NgAgo/gDNA systems to cells and intracellular bacteria in infection sensitive experiments to confirm carefully before using cell lines that are not contaminated or pollution is eliminated" and other details.
On August 8, the site published a report in the journal nature, details of multinational scientists Han Chunyu NgAgo debate. Points out that, from Australia, and Spain, researchers said the experiment may not repeat , while some scientists say Han Chunyu had focussed return experiment, but needs further confirmation.
On the Addgene website, one United Kingdom Centre for regenerative medicine in Edinburgh, a molecular biologist puran·dewarui (Pooran Dewari) published a blog, conducted an online survey on the matter. As of press time, 9 NgAgo claimed in the application system have observed signs of gene shear, 100 people claim that cannot be observed gene shear, 84 expressed the need for further experiments; 9 claimed to observe signs to gene inserts, 47 claims that cannot be observed to gene inserts, 137 people still need further experiment.
It is reported that on May 2, Han Chunyu, Associate Professor of the Hebei University of science and technology in the internationally renowned academic journal articles published in nature biotechnology, and proposed a new method of gene editing--NgAgo. NgAgo are considered existing CRISPR presents challenges, said media reports, this is a "subversive of the fourth generation of gene editing technology". But shortly thereafter, the event into a continuing debate, international scientists question its genome edits cannot be repeated.
On August 2, the journal Nature biotechnology said in a statement, "there are a number of researchers contact the city, unable to carry out this study. We will follow the established process to look into the matter. As a condition of natural science journals of its, authors shall send material, data, code, and related experimental procedures to provide readers, and not be unduly restricted. ”
Beijing, experts in the field of biological sciences, who asked not to be named told reporters that pending the findings of nature biotechnology, who cannot speak Korean false spring, affecting the success of biological science experiment for a number of reasons. "Such a situation is patience magazine survey results. ”
Facing questions, Han Chunyu, told reporters: "no response is the best response. ”
争议不止：韩春雨公开新版详细实验方法，步骤有改动 - 基因,DNA - IT资讯
在韩春雨的舆论风波逐渐发酵之时，8月8日，韩春雨向非盈利性质粒共享信息库Addgene提交新版的详细实验方法，并补充了数项应当注意的问题。
▲图片说明：韩春雨
中国青年报记者在Addgene网站中看到，新版实验方法分为细胞培养、质粒/gDNA共转染、基因组提取三个部分，一些实验细节与其在《自然-生命科学》中发表的论文有所不同。
据“科学美国人”中文版《环球科学》的微信公众号“科普圈”介绍，新实验步骤有几处改变，其中包括“血清品牌由Hyclone变更为Gibco”，“溶解和稀释质粒及gDNA的缓冲液变更为水（pH 8.0）”，“建议在质粒/gDNA共转染的8小时、12小时或24小时后，补转一次gDNA（旧版方法中只提及了‘24小时’）”等内容。
此外，韩春雨还在新版实验方法中列出了4项注意事项，其中包括“NgAgo/gDNA系统对细胞中胞内菌和支原体感染敏感，在实验前要仔细确认所使用的细胞株未被污染或污染已被彻底清除”等细节。
8月8日，《自然》杂志网站发表一篇报道，详细记述了多国科学家对于韩春雨的NgAgo的争论。文章指出，来自澳大利亚、西班牙等国的科研人员表示实验不可重复，另有一些科学家表示曾重复出韩春雨的部分实验，但还需进一步确认。
在Addgene网站上，一名英国爱丁堡再生医学中心的分子生物学家普然·得瓦瑞（Pooran Dewari）发表一篇博客，对此事进行了一次在线调查。截至记者发稿，有9人声称在应用NgAgo系统后观察到了基因剪切迹象，100人声称无法观察到基因剪切，84人表示需要进一步实验；9人声称观察到基因插入迹象，47人声称无法观察到基因插入，137人称还需进一步实验。
据悉，5月2日，韩春雨这位河北科技大学副教授在国际知名学术期刊《自然-生物技术》上发表文章，并提出一种新的基因编辑手段——NgAgo。NgAgo被人认为是对现有的CRISPR技术提出了挑战，有媒体报道时称，这是一个“具有颠覆性的第四代基因编辑技术”。但此后不久，该事件陷入持续性争论，多国科学家质疑其基因组编辑结果无法重复。
8月2日，《自然-生物技术》发表声明称，“已有若干研究者联系本刊，表示无法重复这项研究。本刊将按照既定流程来调查此事。作为在自然科研旗下期刊发表论文的条件之一，作者须将材料、数据、代码和相关的实验流程及时向读者提供，不可加以不当限制。”
北京一位不愿透露姓名的生物科学领域专家告诉记者，在《自然-生物技术》得出调查结果之前，谁也不能说韩春雨造假，影响生物科学实验的成功与否有很多原因。“这样的情况还是耐心等杂志的调查结果吧。”
面对质疑，韩春雨告诉记者：“不回应就是最好的回应。”